isolation and identification of listeria monocytogenes pdf

Isolation and identification of listeria monocytogenes pdf

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Published: 19.05.2021

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Usman, J. Kwaga, J. Kabir, O. Olonitola, S. Radu, F.

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Biological hazards in food View all 26 Articles. Listeria monocytogenes , a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. There are a number of enrichment and plating media that can be used for the isolation of L. Many plating media are available for the isolation of L.

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Listeria monocytogenes is an intracellular pathogen responsible for listeriosis, a foodborne disease that can lead to life-threatening meningitis.

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The present study was undertaken with the prime objective of isolating and identifying Listeria spp. Total samples comprising of milk, milk products, meat, and fish 50 each collected aseptically from local market which were subjected first to pre-enrichment in half strength Fraser broth followed by enrichment in full strength Fraser broth and subsequent plating on PALCAM agar.

1 Introduction

Hansbro, Methods for the isolation and identification of Listeria spp. Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h.

Authors: Anthony D. Hitchins ret. The genus Listeria contains 6 species: L. A taxonomic review of the genus by Rocourt 41 in updates the previous reviews 11 , In recent years, many new species were proposed. However, these new species are not widely adopted and the number of type strains for the newly proposed species are very limited.

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3 comments

  • Leo U. 21.05.2021 at 01:14

    Listeria monocytogenes is one of the most important foodborne pathogens and is a causal agent of listeriosis in humans and animals.

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  • Getacide 24.05.2021 at 20:55

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  • Yeray S. 26.05.2021 at 05:56

    Abstract. Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification tradit.

    Reply

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