recording and controlling the 4d light field in a microscope using microlens arrays pdf

Recording and controlling the 4d light field in a microscope using microlens arrays pdf

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Recording and controlling the 4D light field in a microscope

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Recording and controlling the 4D light field in a microscope

This website uses cookies to deliver some of our products and services as well as for analytics and to provide you a more personalized experience. Click here to learn more. By continuing to use this site, you agree to our use of cookies. We've also updated our Privacy Notice. Click here to see what's new. The light field is a four-dimensional function representing radiance along rays.

In vivo imaging. Fourier transforms. Image resolution. Video acceleration. Charge-coupled devices. Computer simulations.

We explore the use of inexpensive consumer light- field camera technology for the purpose of light-field mi- croscopy. Our experiments are based on the Lytro first gen- eration camera. Unfortunately, the optical systems of the Lytro and those of microscopes are not compatible, lead- ing to a loss of light-field information due to angular and spatial vignetting when directly recording microscopic pic- tures. We therefore consider an adaptation of the Lytro op- tical system. We demonstrate that using the Lytro directly as an oc- ular replacement, leads to unacceptable spatial vignetting. However, we also found a setting that allows the use of the Lytro camera in a virtual imaging mode which prevents the information loss to a large extent. We analyze the new vir- tual imaging mode and use it in two different setups for im- plementing light-field microscopy using a Lytro camera.

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. We explain how volumetric light-field excitation can be converted to a process that entirely avoids 3D reconstruction, deconvolution, and calibration of optical elements while taking scattering in the probe better into account.

This website uses cookies to deliver some of our products and services as well as for analytics and to provide you a more personalized experience. Click here to learn more. By continuing to use this site, you agree to our use of cookies. We've also updated our Privacy Notice. Click here to see what's new. We present a camera array based light field microscopy that utilizes a camera array to simultaneously capture the different perspectives formed by a two-stage relay system.

Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. By inserting a microlens array at the intermediate image plane of an optical microscope, one can record four-dimensional light fields of biological specimens in a single snapshot. Unlike a conventional photograph, light fields permit manipulation of viewpoint and focus after the snapshot has been taken, subject to the resolution of the camera and the diffraction limit of the optical system. Save to Library. Create Alert. Launch Research Feed.

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Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Bimber , Austria Published Computer Science. Light-field microcopy LFM supports single-shot volumetric recording and instant 3D excitation.

Angular control over illumination using our prototype light field illumination system. The specimen is a blond human hair. Scale bar is u. At top are the patterns displayed on our video projector.

Skip to Main Content. A not-for-profit organization, IEEE is the world's largest technical professional organization dedicated to advancing technology for the benefit of humanity. Use of this web site signifies your agreement to the terms and conditions. Aperture interference and the volumetric resolution of light field fluorescence microscopy Abstract: Light field microscopy LFM is an emerging technique for volumetric fluorescence imaging, but widespread use is hampered by its poor spatial resolution.

3 comments

  • Skirretphipong 07.06.2021 at 03:07

    Summary By inserting a microlens array at the intermediate image plane of an optical microscope, one can record four‐dimensional light fields.

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  • Summer1977 08.06.2021 at 06:18

    Request PDF | Recording and controlling the 4D light field in a microscope using microlens arrays | By inserting a microlens array at the.

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